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JUAN FERRER CASANOVA

Juan FerrerPERSONAL DATA

Senior Lecturer
Departament of Agrochemistry and Biochemistry
E-mail: jferrer@ua.es
Telephone: 965909582

Brief summary of work: I focused my research on the characterization and
modifying enzymes of extremophiles from hypersaline environments.

Abridged CV

UNIT OF INVESTIGATION 1Juan Ferrer research group

Structural and molecular analysis of organisms.

LINES OF INVESTIGATION
  • Enzyme Engineering
  • Characterization and Modification of enzymes.
  • Structure and dynamics of proteins.
  • Design of proteins and molecular modeling.
  • Sources of enzymes:
                                 
                    Extremophiles:  Haloferax mediterranei
                                            Halomonas elongata
                                           
    Paenibacillus aquariensis

GROUP OF INVESTIGATION

Proteins of Extremophiles

 PROJECTS OF RECENT INVESTIGATION
  • “Ingeniería de Enzimas” (VIGROB046). Universidad de Alicante. Investigador responsable: Juan Ferrer Casanova. Duración: 01/09/2007-31/08/2009.
  • “Ayuda 2007 para utilización de los SSTTI para estudios estructurales de la enzima 2- hidroxiácido deshidrogenasa de Hf. Mediterraneo y otros” (UAUSTI0701). Universidad de Alicante. Investigador responsable: Juan Ferrer Casanova. Duración: 13/03/2007-12/03/2008.
  • “Red Nacional de Organismos Extremófilos” (BIO200627047E).Ministerio de Educación y Ciencia. Investigador responsable: Mª José Bonete Pérez. Duración: 01/10/2006-30/09/2008.
  • “Estudios estructurales de la especificidad de sustrato en la 2-D-Hidroxiácido deshidrogenasa halofílica” (GV05/166). Consellería de Empresa Universidad y Ciencia. Investigador responsable: Juan Ferrer Casanova. Duración: 01/01/2005-31/12/2006.
  • “Utilización de marcadores moleculares en la determinación de la variedad de la almendra” (ACOMP06/010). Consellería de Empresa Universidad y Ciencia. Investigador responsable: Mª José Bonete Pérez. Duración: 01/01/2006-31/12/2006.
  • “Transferencia de genes implicados en osmoprotección en Halomonas elongata a plantas de interés agrícola." (GV01446). Consellería de Presidencia de la Generalitat Valenciana. Investigador responsable: Juan Ferrer Casanova. Duración: 01/01/2002-31/12/2003.


Private projects:

  • “Análisis químico-biológico de aguas”. Pascual García Gómez & Murray International, S.L. Investigador responsable: Juan Ferrer Casanova. Duración: 30/07/2007-30/09/2011.
  • “Control analítico de aguas”. Pascual García Gómez & Murray International, S.L. Investigador responsable: Juan Ferrer Casanova. Duración: 15/07/2004-02/07/2007.

RECENT PUBLICATIONS

UA Institutional Repository: http://rua.ua.es/dspace/handle/10045/162?locale=en


Book chapters:

  • Bonete, M.J.; Ferrer, J.; Camacho, M.; Pire, C.;  Esclapez, J.; Marhuenda-Egea, F.; Martínez-Espinosa, R.M.; Díaz, S.; Llorca, F.; Pérez-Pomares, F.; Bautista, V. (2005). Enzymes from Halophilic Archaea. EN: Micoorganisms for Industrial Enzymes and Biocontrol / Encarnación Mellado Durán and José Luis Barredo (Editors) / Research Signpost. Pp: 1-24. ISBN: 81-308-0040-3.


Articles in Journals:

  • J.Domenech; J.M. Nieto and J. Ferrer (2009). Behaviour in different denaturant agents and structural characterization by fluorescence techniques of Haloferax mediterranei D-2-hydroxyacid dehydrogenase. Journal of Molecular Catalysis B-Enzymatic 61: 168-173.
  • Domenech, J.;Baker, P.J.;Sedelnikova, S.E.;Rodgers, H.F.;Rice, D.W.; Ferrer,J. (2009). Crystallization and preliminary X-ray analysis of D-2-hydroxyacid dehydrogenase from Haloferax mediterranei. Acta Crystallographica Section F Structural Biology and Crystallization Communications F65: 415-418.
  • Baker,P; Britton, K.; Fisher, M.; Esclapez, J.; Pire, C.; Bonete, M.J.; Ferrer, J.; Rice, D.W. (2009). Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily. Proceedings of The National Academy of Sciences of The United States of America 106 (3): 779-784.
  • Pire, C.; Esclapez, J.; Díaz, S.; Pérez-Pomares, F.; Ferrer, J.; Bonete, M.J. (2009). Alteration of coenzyme specificity in halophilic NAD(P) glucose dehydrogenase by site-directed mutagenesis. Journal of Molecular Catalysis B-Enzymatic 59 (4): 261-265.
  • Esclapez, J.; Pire,C; Bautista, V.; Martinez-Espinosa, R.; Ferrer, J.; Bonete, M.J. (2007). "Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis". FEBS Letters 581 (5): 837-842.
  • Díaz,S.; Pérez-Pomares, F.;  Pire, C.; Ferrer, J. and Bonete, M.J. (2006). Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei. Extremophiles 10 (2): 105-115.
  • Britton, K.; Baker, P.; Fisher, M.; Ruzheinikov, S.; Gilmour, D.; Bonete. M.J.; Ferrer, J.; Pire, C.; Esclpaez, J.; Rice, D. (206). Analysis of Protein solvent interactions in Glucose Dehydrogenase from the extreme halophile Haloferax mediterranei. Proceedings of The National Academy of Sciences of The United States of America 103 (13): 4846-4851.
  • Domenech, J. and Ferrer, J. (2006). A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression. Biochimica Et Biophysica Acta-General Subjects 1760: 1667-1674.
  • Esclapez, J.; Bonete, M.J.; Camacho, M.; Pire, C.; Ferrer, J.; Bautista, V.; Martínez-Espinosa, R.M.;  Zafrilla, B.; Pérez-Pomares, F.; Díaz,S. (2006). An optimized method to produce halophilic proteins in Escherichia coli. Microbial Cell Factories 5 (Suppl 1): S22.
  • Esclapez, J.; Britton, L.K.; Baker, P.J.;  Fisher, M.;  Pire, C.; Ferrer, J.; Bonete, M.J.; and Rice, D.W. (2005). Crystallization and preliminary X-ray analysis of binary and ternary complexes of Haloferax mediterranei glucose dehydrogenase. Acta Crystallographica Section F Structural Biology and Crystallization F61 (8): 743-746.

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Universidad de Alicante
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